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KMID : 0880220100480020184
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Journal of Microbiology
2010 Volume.48 No. 2 p.184 ~ p.187
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Sequence Analysis of the Gene Encoding H Antigen in Escherichia coli Isolated from Food in Morocco
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Samira Badri
Aziz Fassouane
Ingrid Filliol
Mohammed Hassar
Nozha Cohen
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Abstract
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In order to develop other molecular method useful for typing of motile and non motile Escherichia coli strains, a total of 207 strains of E. coli (133 reference strains, 74 food strains) were characterized by analysis of sequences of their amplified flagellin-encoding (fliC) gene products. The collection of reference strains was used for database building of fliC gene sequences. Application of this identification system to 74 E. coli food isolates revealed a reproducible and clear cut classification with very good correlation to results obtained by HhaI restriction of the amplified flagellin gene. The proposed determination of fliC sequences variations should be helpful for epidemiological studies.
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KEYWORD
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E. coli, H-antigen, fliC gene, sequence analysis, PCR-RFLP
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